Characterization of equine butyrylcholinesterase disposition in the mouse.

Characterization of butyrylcholinesterase antagonism of cocaine-induced hyperactivity.

Although there are several published demonstrations that exogenous butyrylcholinesterase (EC 3.1.1.8) works to antagonize cocaine in vivo, a systematic characterization of the enzyme-drug interaction is lacking as is confirmation of the mechanism of effect. This has been addressed using cocaine-induced locomotor activity in mice as a behavioral endpoint. The enzyme was effective, but the enzyme...

Role of oligosaccharides in the pharmacokinetics of tissue-derived and genetically engineered cholinesterases.

To understand the role of glycosylation in the circulation of cholinesterases, we compared the mean residence time of five tissue-derived and two recombinant cholinesterases (injected intravenously in mice) with their oligosaccharide profiles. Monosaccharide composition analysis revealed differences in the total carbohydrate, galactose, and sialic acid contents. The molar ratio of sialic acid t...

Prolonged toxic effects after cocaine challenge in butyrylcholinesterase/plasma carboxylesterase double knockout mice: a model for butyrylcholinesterase-deficient humans.

Death and toxicity after cocaine use do not correlate with cocaine blood levels. One explanation for this observation is that cocaine abusers may posses one or more of the 58 possible known mutations in the butyrylcholinesterase gene (BCHE). Butyrylcholinesterase (BChE) serves as the primary cocaine hydrolase producing a nontoxic product ecgonine methyl ester. A reduction in endogenous levels o...

Large Deformation Characterization of Mouse Oocyte Cell Under Needle Injection Experiment

In order to better understand the mechanical properties of biological cells, characterization and investigation of their material behavior is necessary. In this paper hyperelastic Neo-Hookean material is used to characterize the mechanical properties of mouse oocyte cell. It has been assumed that the cell behaves as continuous, isotropic, nonlinear and homogenous material for modeling. Then, by...

Characterization of the Properties of Mouse-Adapted Human Measles Virus (34333)